Lihlahisoa
2- [tris (hydroxymes)-lithylamino
![2-[Tris(hydroxymethyl)methylamino]-1-ethanesulfonic acid 7365-44-8](http://www.pengnuochemical.com/uploads/2-Trishydroxymethylmethylamino-1-ethanesulfonic-acid-7365-44-8.png)
Synonyms: Chebi: 2 - {[Hydroxy-1,1-Bis (HydroxeMethyl) Methyl-2-Ammoniothate
Theko ea lik'hemik'hale ea 2- [hydroxyMethyl)-methylamino] -1-Ethaudulfenic acid
● Tlhaho / 'mala: White Crystalline Powder
● Lintlha tsa Meli: ~ 223-225 ° C
● index ea theko e tlaase: 1.57
● Pka: 7.5 (e 25 ℃)
● PSA: 135.47000
● Lebaka: 1.554 g / cm3
● Logp: -1.34880
● Temp ea polokelo ea polokelo.
● Fuldubility.:H2O: 1 m oa c, Hlakileng, e hlakile, e se nang mebala
● Scobility ea metsi .: Full
● 1 ea xlogp3: -5.8
● Saydrogen Kamoreng ea Libapali tsa Hydrogen
● Saydrojen
● Palo e potolohileng bond: 6
● Bongata bo Nemahetseng: 229.06200837
● Palo ea atom: 14
● Ho rarahana: 220
Tlhahisoleseling ea Safty
● Ke Pictogram (S):
Xi
● Likhoutu tsa kotsi: Xi
● Lipolelo: 36/37/38
● Lipolelo tsa polokeho: 22-24 / 25-36-26
E na le thuso
● Cannocal oa bososela:C (CS (= O) (= O) [O)) [NH2 +] c (CO) (CO) CO
● Tšepa:Setifikeiti sa ho truris buffer. Biological buffer. TEN ke mofani oa dikarolo tse sebelisoang hangata.
Kenyelletso e qaqhileng
2- [tris (hydroxyMethyl)-methylamino] -1-Ethaudulfenic acidHape, hape e tsejoang e le tem kapa hydroxethylpkyhlPiperazine-n'-2-Etha Aulom e sebelisoang ka biochemical le molek'hule.
TelaHangata e sebelisoa e le buffer ea liteko tsa laboratori ka lebaka la bokhoni ba eona ba ho boloka tikoloho e tsitsitseng, haholo-holo maemong a 6.5 ho isa ho 8.5. E boetse e na le monyaka o tlase oa ho amohela leseli la Ultraviolet, e etsang hore e tšoaneloe ke karabelo e ngata ea Spectrophotometri.
Telae sebelisoa hangata ho litlolo tsa tlhaho le biochemical ho itlhoekisa, proteli
Ho bohlokoa ho hlokomela hore ho bohlokoa haholo ho tsoa tikolohong, kahoo ho bohlokoa hore e boloke le ho e sebetsa ka nepo ho boloka katleho ea eona.
Kopo
2- [Trandro Tse ling tsa ts'ebeliso ea eona ea bohlokoa ke:
Moemeli oa Buffer:Ho sebelisoa tlhahlobo hangata e le motsoako o mong oa biochemical le molek'hule ea biolocular ea biogymical le molek'hule. E thusa ho boloka tikoloho e tsitsitseng ea pH, haholo-holo maemong a 6.5 ho isa ho 8.5
Likarolo tsa koetliso ea tlhaho le biochemical:Ho na le tlhahlobo e sebelisoang ka tsela e fapaneng ea likokoana-hloko le biochemical moo ho boloka pH ka liphetho tse ntle ke tsa bohlokoa. E thusa ho boloka PH ea tharollo ea 'nete le ho khothatsa botsitso maemong a karabelo.
PUSELETSO EA Protroin:Ho sebelisoa tlhahlobo khafetsa tsamaisong ea prothein ka lebaka la bophahamo ba eona ba budffee le ho ikamahanya le tšebetso ea enzyme. E thusa ho boloka botsitso le tšebetso ea liprotheine nakong ea mehato ea tlhoekiso.
Electrophoresis:Ho hlahlojoa ho sebelisoa e le motsoako oa buffring ho gel Electrophoresis, haholo-holo Polycerlaymide Gel Electrophoresis (leqephe). E fana ka tikoloho e tsitsitseng ea pherekano ea protheine le ho falla.
Media Media Media:Ho hlahlojoa ho kenyelelitsoe ho Media Media Media ho boloka PH e sa feleng bakeng sa kholo ea sele e nepahetseng le ho ata. E thusa ho tsitsisa pH ea setso sa setso ebile e fana ka tikoloho e loketseng lisele.
Ho bohlokoa ho hlokomela hore ts'ebeliso e tobileng le khatello ea maikutlo e ka fapana ho latela litlhoko tsa liteko. Ho sebetsana ka nepo, ho boloka le ho bona lintlha tse boletsoeng litokomaneng tsa theknoloji ea sehlahisoa li bohlokoa ho netefatsa ts'ebetso ea eona ea polokeho le polokeho.
