Lihlahisoa
Hepes; CA.7365-45-9
Synonyms: 1- (4- (hydroxyethyl) -1-pimperous] ethane-2-sulfsonic et acid); n- (2-hydroxethyl) Piperazine-N'. acid); n'-y-hydroxethylpimperazine-n-hydropulfenic acid) -;
Thepa ea lik'hemik'hale ea Hepes
● Tlhaho / 'mala: White Crystalline Powder
● khatello ea mouoane: 0PA ho 25 ℃
● Taba ea ho khetholla: 235-238 ° C
● Indrantive e re: N20 / D 1.339
● Khoeli ea ho pheha: 408 ℃ [ho 101 325 pa]
● Pka: 7.5 (e 25 ℃)
● PASA:89.46000
● Lebaka: 1.325 g / cm3
● Logp: -0.55930
● Temp.Un °.N.
● Fuldubility.:H2O: 1 m oa c, Hlakileng, e hlakile, e se nang mebala
● Scobity.Olulleble
● 1 ea xlogp3: -4.1
● Saydrogen Kamoreng ea Morekisi ea Hydrogen
● Saydrogen oamohelo oa Hydrogen
● Palo e khutsitseng e potolohang: 4
● Ho na le boima bo nepahetseng: 238.09872833
● Ethe ea atom: 15
● Ho rarahana: 254
Tlhahisoleseling ea Safty
● Ke Pictogram (S):
Xi
● Likhoutu tsa kotsi: Xi
● Lipolelo: 36/37/38
● Lipolelo tsa polokeho: 24 / 25-22-36-26
E na le thuso
Cannonical oa bososela:C1cn (cc [NH + leqepheng la (= O) (= = O) [o -]) CCO
Tlhaloso:Hepes e hlalositsoe e le e 'ngoe ea li-buffls tse ntle ka ho fetisisa tse fumanehang bakeng sa lipatlisiso tsa tlhaho. Ho Bioloji PH, molek'hule ke ZWFIANIC, 'me ea sebetsa joaloka buffer ho PH 6.8 ho isa ho 8.2 (Pka 7.55). Hangata e sebelisoa ka moetlo oa sele ho tsepamiso lipakeng tsa 5mm ho 30 mm. Hepes e sebelisitsoe likopatsong tse fapaneng, ho kenyeletsoa moetlo oa lisele. E tloaelehile ho sebelisa media ea sele e mecha ea lihloliloeng moeeng. Mehama e fumana tšebeliso ea tsona ho Vitro liteko tsa vitro ho MG.
Ts'ebeliso:Eseng chefo ho sele. E sebelisoa e le buffer ea hydrogen ion buffer ea hydrogen, e ka laolang mofuta oa ph o sa khaotseng nako e telele. Khokahano ke 10-50mmol / l. Ka kakaretso, 20mmol / Lihepes ka tharollo e ka fumana bokhoni ba buffer. Biological buffer. Metepe e tloaelehileng ea mahlale a tlhaho, haholo sebelisitsoe haholo moetlong oa sele ho boloka ph. E nka karolo ea bufflering, e sebelisoang ho lokisoa ha ba buffer. E hlalositsoe e le e 'ngoe ea li-buffls tse ntle ka ho fetisisa tse fumanehang e le karolo ea mofuta oa tlhaho, tse sebelisoang ho hlophisoa ha litlolo tsa lehae.
Kenyelletso e qaqhileng
Ma hepee emetse 2- (4- (2- (2 - hydroxethyl) Pipelin-1-YL) Etha Aunesfulfenic Acid. Ke moemeli ea busang hangata a sebelisoang ho etsa lipatlisiso tsa tlhaho le biochemical. Heroes e thusa ho boloka pH e tsitsitseng ho media mecheng ea selefouno, joalo ka ha e khona ho amohela le ho fana ka litlatsetso, ka ho etsa joalo e laoloa liphetoho. Its chemical structure and properties make it highly effective in maintaining a constant and optimal pH for many biological and enzymatic reactions. Mehopolo e sebelisoa hangata moetlong oa sele, pro-Propein, le mekhoa ea biology ea biology ea biology.
Kopo
Metero e na le likopo tse 'maloa masimong a fapaneng a lipatlisiso. Tse ling tsa lits'ebetso tse tloaelehileng tsa li-hepes ke:
Setsi sa sele: Metsu e sebelisoa e le karolo ea media mecheng ea Cell ho boloka tikoloho e tsitsitseng ea pH. E thusa laola hore phallo e be teng ke lintho tse bakoang ke lintho tsa metabolic le pokello ea lihlahisoa tsa litšila likarolong tsa litso tsa sele.
Enzyme Assays:Metepe e sebelisoa e le moemeli oa bubang e sebelisana le Enzys o sebelisa ph e sa sebetseng hantle, e bohlokoa bakeng sa ts'ebetso e nepahetseng ea li-enzyme. E thusa ho netefatsa sephetho se nepahetseng le se khothatsang.
Electrophoresis:Metero e sebelisoa e le moetsi oa litheko ho electrophoresis joalo ka polyacrylamide Gel Electrophoresis (leqephe) le Agarose Gel Electrophoresis. E thusa ho boloka buka e lakatsehang bakeng sa karohano e nepahetseng le ho hlahlojoa ha macroromolecules e joalo ka liprotheine le li-acid acid.
PUSELETSO EA Protroin:Metepo e kenyelelitsoe lithepe tsa prothein tsa prothein ho boloka phallo e lakatsehang nakong ea ts'ebetso e fapaneng ea tlhoekiso. E thusa ho boloka botsitso le tšebetso ea liprotheine nakong ea mehato ea ho tšoatsoa.
Mekhoa ea biogy ea biology ea molegy:Li-hepe li ka sebelisoa ho mekhoa e fapaneng ea biogy, e kang pcr (polymerase Chain recection) le DNA e latellanang, ho boloka pH e tsitsitseng. E thusa ho ntlafatsa ts'ebetso ea li-enzyme le ho netefatsa litholoana tse nepahetseng le tse tšepahalang.
Ho bohlokoa ho hlokomela hore khatello e tobileng ea mathepe le ts'ebeliso ea eona e ka fapana ho latela maemo a liteko le litlhokahalo.
