Lihlahisoa
HEPES letsoai la sodium
Litlhaloso tse tšoanang:HEPES letsoai la sodium;75277-39-3;HEPES hemisodium letsoai;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt;4-(2-Hydroxyethyl)piperazine-1- letsoai la ethanesulfonic acid; sodium; 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:4675; 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium letsoai (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium letsoai;HEPES sodium letsoai, > = 99.5% (titration); sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) letsoai la sodium;MFCD00036463;HEPES, sodium letsoai;HEPES sodium letsoai, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-OPE-S letsoai.Na; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium letsoai, biochemical kereiti;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-061030863;6F8;6F8 N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium letsoai, BioXtra, >=99.0% (titration);HEPES sodium letsoai, Vetec(TM) reagent grade, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl) -1-piperazineethanesulfonic acid letsoai la sodium;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium letsoai;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium letsoai;HEPES letsoai la sodium, BioPerformance Certified, e loketseng setso sa lisele, =99.5%
Thepa ea lik'hemik'hale ea HEPES Sodium Letsoai
● Ponahalo/Mmala: phofo e tšoeu
● Khatello ea Mouoane: 0Pa ho 25℃
● Sebaka se qhibilihang: 234 °C
● PKA:7.5(ho 25℃)
● PSA:92.29000
● Boima ba 'mele:1.504[ho 20℃]
● LogP: -0.90190
● Mocheso oa ho Boloka.: Boloka ho RT.
● E na le kutlo.: Hygroscopic
● Solubility.:H2O: 1 M ho 20 °C, e hlakile, ha e na 'mala
● Ho qhibiliha ha Metsi.: E qhibiliha ka metsing.
● Hydrogen Bond Donor Count:1
● Hydrogen Bond Accepter Count:6
● Potoloho ea Bond Count:5
● Hantle 'Misa: 260.08067248
● Palo ea Athomo e Matla:16
● Ho rarahana:272
Boitsebiso bo Boreleli
● Litšoantšo:
● Likhoutu tsa Kotsi:
● Lipolelo tsa Tšireletseho: 22-24/25
E molemo
Lihlopha tsa Lik'hemik'hale:Lisebelisoa tse ling -> Li-Biological Buffers
Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Lisebelisoa:Tharollo ea letsoai ea HEPES ea letsoai e loketse lithuto tsa setso sa lisele. E ka sebelisoa ho lokisa tharollo ea buffer ea HEPES. E ka sebelisoa e le buffer nakong ea litlhahlobo tsa phosphorylation ho li-fibroblasts tse nang le permeabilized. E ka 'na ea sebelisoa ho etsa sekontiri se rarahaneng sa dialysis, se sebelisetsoang qeto ea thyroxin ea mahala serum ka radioimmunoassay. HEPES sodium salt solution e loketse ho sebelisoa lithutong tsa cell culture. E ka sebelisoa ho lokisa tharollo ea buffer ea HEPES. E ka sebelisoa e le buffer nakong ea litlhahlobo tsa phosphorylation ho li-fibroblasts tse nang le permeabilized. E ka 'na ea sebelisoa ho etsa setlolo se rarahaneng sa dialysis, se sebelisetsoang qeto ea thyroxin ea mahala serum ka radioimmunoassay.
Kenyelletso e Feletseng
HEPES letsoai la sodium, e tsejoang hape e le 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt, ke mofuta o sebelisoang hangata oa HEPES. Ke motsoako oa zwitterionic organic o sebetsang e le sesebelisoa se thibelang lipatlisiso tsa bioloji le tsa biochemical.
HEPES letsoai la sodium le atisa ho sebelisoa mecheng ea litaba tsa setso le likokoana-hloko tsa likokoana-hloko ka lebaka la bokhoni ba eona ba ho boloka pH e tsitsitseng e potolohileng maemo a fisioloji (pH 7.2 - 7.6). E qhibiliha haholo metsing 'me e na le chefo e batlang e le tlase, e etsa hore e tšoanelehe bakeng sa lisebelisoa tse fapaneng.
Mofuta oa letsoai oa sodium oa HEPES o ratoa lits'ebetsong tse ngata kaha o matlafatsa ho qhibiliha le botsitso, ha o bapisoa le foromo ea mahala ea asiti. E fumaneha khoebong e le phofo e tšoeu ea kristale e ka qhibilihang habonolo metsing ho lokisetsa tharollo ea ho sebetsa.
Bafuputsi ba tloaetse ho sebelisa letsoai la sodium la HEPES mekhoeng ea baeloji ea limolek'hule, setso sa lisele, liteko tsa enzyme, tlhoekiso ea protheine, le liteko tse ling tsa biochemical moo taolo ea pH e leng bohlokoa. Bokhoni ba eona ba ho thibela le ho lumellana le lits'ebetso tsa baeloji bo e etsa khetho e tsebahalang bakeng sa ho boloka maemo a nepahetseng bakeng sa ts'ebetso le botsitso ba limolek'hule tse fapaneng tsa tlhaho.
Pele o sebelisa letsoai la sodium la HEPES ts'ebetsong efe kapa efe e khethehileng, ho bohlokoa ho hlahloba lingoliloeng, ho shebana le likhothaletso tsa barekisi, le ho ntlafatsa boemo ba pH ho ipapisitse le litlhoko tse khethehileng tsa liteko.
Kopo
HEPES letsoai la sodium le fumana ts'ebeliso e atileng libakeng tse fapaneng tsa lipatlisiso tsa bioloji le lik'hemik'hale. Tse ling tsa lisebelisoa tsa eona tsa bohlokoa li kenyelletsa:
Cell Culture:HEPES letsoai la sodium le atisa ho eketsoa mecheng ea litaba tsa setso sa lisele ho boloka pH e tsitsitseng ka har'a mefuta ea physiological le ho fana ka tikoloho e loketseng ea hore lisele li hōle le ho ata.
Buffering Agent:HEPES letsoai la sodium hangata le sebelisoa e le ntho e thibelang likokoana-hloko le litharollo, ho kenyeletsoa liteko tsa li-enzyme, tlhoekiso ea protheine le mekhoa ea baeloji ea limolek'hule. E thusa ho boloka pH e sa khaotseng ka ho hanyetsa liphetoho tsa acidity kapa alkalinity.
Electrophoresis: HEPES letsoai la sodium le sebelisoa e le buffer ho gel electrophoresis ho boloka pH e tsitsitseng nakong ea karohano ea nucleic acids kapa protheine. E thibela liphetoho ho pH tse ka amang ho falla le ho arohana ha limolek'hule tsa gel.
Protein Stability: HEPES letsoai la sodium le eketsoa litharollong tsa protheine ho tsitsisa sebopeho sa tsona le ho boloka tšebetso ea tsona. E thusa ho boloka tikoloho e nepahetseng ea pH e hlokahalang bakeng sa botsitso le ts'ebetso ea protheine.
Ts'ebetso ea enzyme: HEPES letsoai la sodium le sebelisoa e le setšireletsi tšebetsong ea enzymatic ho boloka pH e lakatsehang bakeng sa tšebetso e nepahetseng ea enzyme. E thusa ho netefatsa hore li-enzyme li sebetsa ka katleho le ho nepahala ha tsona.
Live Cell Imaging:HEPES letsoai la sodium hangata le kenyeletsoa mecheng ea litšoantšo bakeng sa liteko tsa ho etsa litšoantšo tsa lisele. Bokhoni ba eona ba ho buffer bo thusa ho boloka pH e lakatsehang le ho thibela ho feto-fetoha ho ka amang ho sebetsa le fluorescence ea lisele.
Mekhoa ea Baeloji ea Limolek'hule: HEPES letsoai la sodium le sebelisoa haholo mekhoeng e fapaneng ea baeloji ea limolek'hule, ho kenyelletsa DNA kapa RNA isolation, PCR, tatellano ea DNA, le tlhahlobo ea protheine. Bokhoni ba eona ba ho buffer bo lumella liphetho tse nepahetseng le tse tšepahalang nakong ea lits'ebetso tsena.
Ho bohlokoa ho ela hloko hore ts'ebeliso e khethehileng le kholiso ea letsoai la sodium la HEPES li ka fapana ho latela litlhoko tsa liteko le sistimi ea baeloji e ntseng e ithutoa. Ka hona, ho bohlokoa ho shebana le lingoliloeng le likhothaletso tsa barekisi bakeng sa tšebeliso e nepahetseng.
